玻璃化冷凍平衡(ES)時間9-10分鐘可達最佳懷孕率
玻璃化冷凍平衡時間低於7分鐘懷孕率呈下降

Hum Fertil (Camb). 2016 May 24:1-6. [Epub ahead of print]

Shortened equilibration time can compromise clinical outcomes in human embryo vitrification.

Xiong S1, Liu JX2, Gao Y1, Liu WW1, Wu LH1, Han W1, Zhang XD1, Han SB1, Liu DY1, Huang GN1.

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Abstract

Vitrification is an important way to cryopreserve human embryos and the recommended time of embryo exposure to the vitrification solution is 1 min. However, practically speaking, the duration of embryos exposure to equilibration solution can vary from 5 to 15 min. The purpose of this study was to investigate the effect of different equilibration times on the outcomes of frozen-thawed embryo transfer cycles. The data were collected from our medical records from January 2012 to June 2013 and a total of 517 cycles were included. These cycles were divided into four groups according to the equilibration time: (i) 5-6 min; (ii) 7-8 min; (iii) 9-10 min and (iv) 11-12 min. The results show that there were no differences in terms of survival rate and fully intact embryo rate among the four groups. However, lower clinical pregnancy, embryo implantation and live birth rates were observed in the 5-6 min exposure group (54.6%, 31.9% and 48.2%, respectively) compared with the three other groups. The corresponding rates in the 9-10 min group (73.5%, 47.6% and 64.7%) were the highest. This study indicated that different equilibration times influenced the clinical outcomes of human embryo vitrification and vitrification with shortened equilibration time compromised the clinical outcomes. Appropriate prolongation of the equilibrium time would probably improve the clinical outcomes.