以immunocytochemistry 顯影,  laser confocal microscopy偵測卵子纺綞體&染色體
高齡卵子纺綞體&染色體異常率明顯較高

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Feb;24(1):6-9.

[Influence of maternal age on meiotic spindle and chromosome configuration of oocytes].

[Article in Chinese]

Chen W1, Hu J, Liu Q, Wei YL, Zhu GJ.

Author information

Abstract

OBJECTIVE:

To study the effect of maternal age on meiotic spindle and chromosome configuration of oocytes.

METHODS:

Spindle and chromosome configuration was examined in day 1 unfertilized human oocytes after in vitro fertilization (IVF) andintracytoplasmic sperm injection(ICSI) by immunocytochemistry and visualized by laser confocal microscopy.

RESULTS:

Statistically significant differences were observed on normal spindle and chromosome configurations of oocytes between 25-29 maternal age group (33% and 31%, respectively), and 30-34 age group (P< 0.05) as well as 35-40 age group(0%, P<0.01). The incidence of abnormal spindle and chromosome configurations of oocytes from 30-34 and 35-40 maternal age groups was much higher than that of oocytes from 25-29 age group (P<0.01, P<0.05).

CONCLUSION:

Incidence of abnormal spindle and chromosome configuration of oocytes is related to maternal age. It could be an important reason of age related oocyte aneuploidy.