動物實驗顯示低劑量抗凝劑肝素(Heparin 40 μg/ml=4 IU/ml)使用於IVF精蟲之處理過程有助於精蟲之頂體成熟&進一步胚胎發育與囊胚形成
(100 IU unit of heparin is equivalent to 1mg)

https://www.jstage.jst.go.jp/article/jrd/advpub/0/advpub_2016-142/_pdf

J Reprod Dev. 2017 Feb 5. doi: 10.1262/jrd.2016-142. [Epub ahead of print]

Significant heparin effect on bovine embryo development during sexed in vitro fertilization.

An LY1, Chaubal SA, Liu Y, Chen Y, Nedambale TL, Xu J, Xue F, Moreno JF, Tao S, Presicce GA, DU F.

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Abstract

The aim of this study was to investigate the effect of different heparin concentrations in the course of sexed in vitro fertilization (IVF), on bovine embryonic development and development to term following embryo transfer (ET). With a total of 9156 oocytes for IVF, sorted as well as unsorted sperm from four bulls had different heparin requirements for achieving the highest rate of development in vitro. However, when optimal heparin concentrations were used (40 to 80 µg/ml), the performance of X-sorted sperm (0.3 × 106/ml/IVF droplet) from all four bulls, as judged by blastocyst development (Bulls A, B, C, and D: 25.2, 19.7, 25.1, and 9.8%, respectively), was significantly increased, and the blastocyst rate was comparable to that observed with unsorted sperm at certain heparin concentrations within the four bulls. We determined that near-optimal blastocyst development was possible with sorted sperm from all four bulls, when a heparin concentration of 40 µg/ml was used. Pregnancy rates at d 70 post ET ranged from 39.1 to 40.3% (P > 0.05), and the calving rates ranged from 34.4 to 35.1% (P > 0.05), when heparin was used at a concentration of 10 μg/ml (n = 236), 20 μg/ml (n = 189), and 40 μg/ml (n = 305), respectively. Our study demonstrates that, although the sorted sperm of different bulls performed optimally over a range of heparin concentrations, a generally accepted heparin concentration of 40 µg/ml can be set for sexed IVF. This improvement is beneficial when sexed embryo production by ovum pickup and IVF is an essential component of genetic breeding programs.