精蟲-玻尿酸(hyaluronan, HA)結合力檢驗
sperm–hyaluronan binding assay (HBA)
(A) 精蟲結合到玻尿酸 Spermatozoa bound to the HA spot on PICSI dish
(B) 精蟲以毛細管取出 HA bound spermatozoa being picked up by ICSI pipette
(C) and (D) 精蟲移到高倍顯微鏡觀查
transferred to glass dish for morphological evaluation at high magnification
(A) 正常精蟲 Normal spermatozoa,
(B-D)外型異常精蟲 Abnormalityof nuclearform: spermatozoa with small or large oval nuclear forms
(B), spermatozoa with wide or narrow nuclear forms
(C), spermatozoa with regional shape abnormality of nuclear form (D),
(E-F) 細胞核異常/含空泡之精蟲
Abnormalities in nuclear chromatin: spermatozoa with vacuoles occupying >4-50% of the nuclear area
(E) spermatozoa with large nuclear vacuoles (> 50% of the nuclear area)(F).
結論: 與玻尿酸結合之精蟲vs.未與玻尿酸結合之精蟲 ，
其精蟲型態並無差異
Ref: Reprod Biol Endocrinol. 2010; 8: 149
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