玻璃化冷凍過程病毒傳染之可能性

病患人數: 24
病毒篩檢種類: 愛滋病，B&C形肝炎(HIV, HCV, HVB)
結論:
玻璃化冷凍過程病毒傳染之可能性不高
開放型玻璃化冷凍載具在液態氮低溫冷凍環境似乎並不明顯容易傳染上述病毒



http://www.ncbi.nlm.nih.gov/pubmed/22035968

Fertil Steril. 2011 Oct 26. [Epub ahead of print]

Viral screening of spent culture media and liquid nitrogen samples of oocytes and embryos from hepatitis B, hepatitis C, and human immunodeficiency virus chronically infected women undergoing in vitro fertilization cycles.

Cobo A, Bellver J, de Los Santos MJ, Remohí J.

Source

IVI-Valencia, Institut Universitari IVI, Valencia, Spain.

Abstract

OBJECTIVE:

To assess the presence of viral RNA or DNA sequences in spent culture media used after ovum pickup (OPU) or embryo culture and in liquid nitrogen (LN) used for oocyte or embryo vitrification in patients seropositive for human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) undergoing IVF cycles.

DESIGN:

Descriptive study.

SETTING:

Private university-affiliated IVF center.

PATIENT(S):

Twenty-four women who underwent controlled ovarian stimulation for oocyte vitrification or IVF/ET. A total of 6, 11, and 6 patients were seropositive for HIV, HCV, and HBV, respectively, whereas 1 patient showed a coinfection with HCV and HBV. Seven patients presented positive blood viral load (HIV, n = 1; HBV, n = 1; HCV, n = 5). Sixty-three samples were analyzed: follicular fluid, n = 3; spent culture media, n = 33 (20 after OPU and 13 after embryo culture); and LN, n = 27 (14 and 10 after oocyte and embryo vitrification; and 3 LN storage tank samples).

INTERVENTION(S):

Ovum pickup, oocyte and/or embryo culture, and/or vitrification by the Cryotop open device. Reverse transcription-polymerase chain reaction analysis was performed for viral screening.

MAIN OUTCOME MEASURE(S):

Detection of viral sequences of HIV, HCV, and HVB.

RESULT(S):

All the samples analyzed tested negative for the detection of viral RNA or DNA sequences.

CONCLUSION(S):

We have not detected viral sequences after culture and vitrification of oocytes/embryos from HIV-, HBV-, and HCV-seropositive patients. These findings represent good evidence of the lack of risk of cross-contamination among seropositive patients, even using an open device for vitrification.