卵子異常外觀並不影響玻璃化冷凍後卵子存活率

卵子異常外觀包括:

1. cytoplasmic granularity, 細胞質過多顆粒

2. vacuoles in the ooplasm, 細胞質空泡

3. aggregates of smooth endoplasmic reticulum in the ooplasm,細胞質聚集異常內質網

4. large perivitelline space size, 細胞膜與中空透明帶間空間過大

5. perivitelline space granularity,  細胞膜與中空透明帶間空間異常顆粒結構

6. fragmented first polar body and zona pellucida abnormalities.極體異常外觀或碎片

但卵子異常外觀並不影響玻璃化冷凍後卵子存活率

J Assist Reprod Genet. 2011 Dec 4. [Epub ahead of print]

Oocyte morphology does not affect post-warming survival rate in an egg-cryobanking donation program.

Setti AS, Figueira RD, Braga DP, Ferreira RC, Iaconelli A Jr, Borges E Jr.

Source

Sapientiae Institute - Educational and Research Centre in Assisted Reproduction, Rua Vieira Maciel, 62, 04203-040, São Paulo, SP, Brazil.

Abstract

PURPOSE:

To evaluate whether oocyte dysmorphisms affect oocyte survival rates in an egg-cryobanking donation program.

METHODS:

This study included 54 patients undergoing intracytoplasmic sperm injection. A total of 415 metaphase II oocytes were vitrified using the Cryotop method. Oocyte morphology was assessed immediately prior to oocyte vitrificationunder 400× magnification. The influence of dysmorphisms on post-thaw survival rates was assessed using regression analysis. Results were considered to be significant at the 5% critical level.

RESULTS:

Oocyte survival rate was not affected by the presence of the following analysed oocyte abnormalities: increased cytoplasmic granularity, vacuoles in the ooplasm, aggregates of smooth endoplasmic reticulum in the ooplasm, large perivitelline space size, perivitelline space granularity, fragmented first polar body and zona pellucida abnormalities.

CONCLUSIONS:

Oocyte morphology, observed prior to vitrification, does not predict post-warming survival. The non-invasive identification of predictive markers for oocyte survival potential remains a difficult task.