胚葉細胞之細胞核數量可評估胚胎染色體之正常機率

胚葉細胞之細胞核數量可評估胚胎染色體之正常機率
具單一細胞核胚葉細胞之胚胎其染色體正常機率較高
同時其著床率與懷孕率亦較高


from: http://www.ncbi.nlm.nih.gov/pubmed/21851938

Fertil Steril. 2011 Oct;96(4):856-9.

Multinucleation of a sibling blastomere on day 2 suggests unsuitability for embryo transfer in IVF-preimplantation genetic screening cycles.

Ambroggio J, Gindoff PR, Dayal MB, Khaldi R, Peak D, Frankfurter D, Dubey AK.

Source

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, George Washington University, Washington, DC 20037, USA.

Abstract

OBJECTIVE:

To evaluate the impact of multinucleation of a sibling blastomere of day 2 embryos on the rate of aneuploidy detected by day 3 preimplantation genetic screening (PGS) biopsy and the effect on subsequent implantation and pregnancy rates.

DESIGN:

Retrospective cohort study.

SETTING:

University-based IVF center.

PATIENT(S):

A total of 141 couples undergoing their first IVF-PGS cycle for idiopathic recurrent pregnancy loss (RPL) or multiple failed IVF implantations.

INTERVENTION(S):

Biopsy of single-nucleated blastomeres for PGS analysis of chromosomes X, Y, 13, 15, 16, 17, 18, 21, and 22 by fluorescence in situ hybridization.

MAIN OUTCOME MEASURE(S):

Aneuploidy, implantation, and pregnancy rates.

RESULT(S):

PGS revealed an increased incidence of aneuploidy when comparing multinucleated day 2 embryos with single-nucleated embryos (85% vs. 78%; relative risk 0.92 (95% confidence interval 0.84-1.00). Transfer of single-nucleated euploid embryos resulted in clinical pregnancy and implantation rates of 28% and 24%. Transfer of multinucleated euploid embryos resulted in no implantations.

CONCLUSION(S):

The presence of multinucleated blastomeres on day 2 of embryo development, 1 day before biopsy, predicts an increase of aneuploidy and poor pregnancy outcomes in IVF-PGS cycles.