藉此可開發出更高懷孕率之培養液與冷凍解凍液
排出1st PB後仍需1-2h卵子才會完全進入MII
取卵後3-4h施行ICSI較1-2h為妥當
polescope可用於偵測精子頭部頂體反應acrosome reaction,藉以挑選優質精蟲
http://humupd.oxfordjournals.org/content/17/5/654.full
Figure 3
Spindle dynamics during the meiotic cell cycle. The course of the meiotic cell cycle in a human oocyte is shown from the beginning of the extrusion of the first polar body up to the dissolution of the spindle. The images represent a total time scale of 160 min (A–I) and the time difference from one image to the following is 20 min. Note the increase in spindle birefringence during the phase of polar body extrusion (A–C) and the presence of a spindle bridge, which connects the first polar body to the ooplasm for another 60–80 min before the spindle is completely dissolved (D–I). Pictures were taken out of a video sequence which is available online as Supplementary data.
Figure 4
Zona imaging using automatic zona detection and analysis. The inner layer of the zona pellucida can be visualized by polarizing microscopy. An automatic zona imaging system (Octax PolarAide, Octax, Bruckberg, Germany) allows for the generation of a score which is mainly based on the intensity and uniformity of the zona birefringence. The oocytes displayed show either a very uniform and strong birefringence (4A, score high = green), a less uniformity and birefringence (4B, yellow = intermediate) and a very low and extremely irregular birefringence (4C, red = score low) of the inner zona layer. A video sequence is available online (Supplementary data), which shows the detection of the zona pellucida birefringence by an automatic module.
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