oocyte成熟過程
GVBD>Metaphase I(M1)>Anaphase I>Telophase I>Metaphase II(M2)
使用polescope可分辨telophase & metaphase II
少女初經時有30萬顆卵細胞,終其一生僅約650顆卵子成熟排出,
male PN 比female PN 較靠近 PB
male PN比female PN略大
http://humupd.oxfordjournals.org/content/14/5/431.full
Figure 1:
Representative images composed by the authors of immunocytochemically stained oocytes at various meiotic stages to demonstrate chromatin and microtubule dynamics during oocyte nuclear maturation.
These images serve to reinforce the fact that simple visualization of polar body extrusion does not indicate fidelity of oocyte maturation, as unseen chromosomal or meiotic spindle defects may be present. Microtubules are stained with β-tubulin (green), while chromatin is stained with Hoescht (blue). GVBD and metaphase I images were taken at ×100. Anaphase, telophase and metaphase II images were taken at ×93.
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