取卵針取卵壓力控制應以低壓180mmHg為適當

以20-gauge取卵針取卵壓力控制應以低壓180mmHg為適當
180mmHg取出之卵子比300mmHg取出之卵子具有較高之懷孕率

http://www.ncbi.nlm.nih.gov/pubmed/17425825

Reprod Biomed Online. 2007 Apr;14(4):444-9.

Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle.

Hashimoto S, Fukuda A, Murata Y, Kikkawa M, Oku H, Kanaya H, Sonoda M, Sugihara K, Murata T, Nagata F, Nakaoaka Y, Morimoto Y.

Source

IVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015 Japan. hashimoto@ivfnamba.com

Abstract

In-vitro maturation (IVM) of immature oocytes has been proposed as a potential alternative to conventional IVF treatment following ovarian stimulation. However, the effects of the oocyte retrieval conditions on subsequent development have not been well understood. This study assessed the effects of different aspiration vacuums during oocyte retrieval on the developmental competence of immature oocytes following IVM, IVF and embryo transfer, retrospectively. Immature oocytes were aspirated with 20-gauge needles with a vacuum of 180 or 300 mmHg. Immature oocytes were cultured in IVM medium for 26 h. All mature oocytes were inseminated by intracytoplasmic sperm injection (ICSI). Embryo transfer was carried out 2 or 3 days after ICSI. The percentage of cumulus-cell enclosed oocytes and of transferable embryos per retrieved oocytes in 180 mmHg (69.7% and 23.8%, respectively) were significantly higher (P < 0.01) than those in 300 mmHg (46.2% and 12.8%, respectively). The ongoing pregnancy rate per retrieval cycle in 180 mmHg (30%) was higher (P < 0.01) than that in 300 mmHg (4.3%). The data indicate that lower pressure of vacuum aspiration with a 20-gauge needle improves the developmental competence of immature oocytes following IVM, IVF and embryo transfer.