人類胚胎幹細胞培養

使用Mouse Embryonic Fibroblast Feeder Cells之培養基用於培養人類胚胎幹細胞

人類胚胎幹細胞可用於藥物測試, 基因檢測, 等實驗


http://cshprotocols.cshlp.org/content/2008/9/pdb.prot5044.full


Culturing Human Embryonic Stem Cells in Feeder-Free Conditions

1. Sohyun L. McElroy1 and 
2. Renee A. Reijo Pera

+Author Affiliations

1. Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA

1. ↵¹Corresponding author (sohyun@stanford.edu)

 

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INTRODUCTION

Human embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs without feeder cell contamination.

Figure 1.

(A) A small undifferentiated hESC colony on day 1 after passage on a Matrigel-coated plate. (B) A single hESC colony ready for passage. Images were captured at 50X magnification.