玻璃化冷凍封閉型載具比開放型載具較易造成胚胎損傷

玻璃化冷凍封閉型載具比開放型載具因溫度下降上升較慢，較易造成胚胎冷凍解凍過程之損傷
囊胚期玻璃化冷凍vs. 8-cell期玻璃化冷凍，囊胚期玻璃化冷凍DNA損傷率較高
液態氮蒸氣保存與液態氮保存效果類似

http://www.biomedcentral.com/1472-6750/11/29

Resolution: standard / high Figure 3. Cleavage and blastocyst stage embryos were vitrified in different carriers and stored in liquid nitrogen (LN) or held in the vapor phase (VP) of a liquid nitrogen dry shipper for 96 hours to simulate transport conditions. Upon warming, DNA damage was assessed by quantification of the percentage of blastomeres per embryo exhibiting DNA fragmentation. The only carrier to exhibit a difference was the Cryotip, with a significantly higher percentage of DNA damage with blastocyst storage in the liquid phase. *P = 0.004. The percentage of DNA damage was significantly higher with vitrification of blastocysts versus cleavage stage embryos (P < 0.001). AbdelHafez et al. BMC Biotechnology 2011 11:29   doi:10.1186/1472-6750-11-29 Download authors' original image