第一極體排出後2-4小時施予ICSI可達最佳受孕率

第一極體(PB)排出後約2-4小時施予ICSI可達最佳受孕率
第一極體(PB)排出後1小時內，卵細胞核未達成熟狀態可施與ICSI

http://humrep.oxfordjournals.org/content/22/7/1991.long

Table 1:

The fertilization and good embryo^a development rates after ICSI of IVM MI → first PB and IVM (control) human oocytes in HCG-primed IVM cycles

Oocytes	ICSI time post-first PB extrusion	Total no. of oocytes	2PN (%)	No. of good quality embryos (%)
MI → first PB	<1 h	38	6 (15.8)*	2 (33.3)
	1–2 h	30	24 (80.0)	8 (33.3)
	2–4 h	26	24 (92.3)	12 (50.0)
	4–6 h	28	23 (82.1)	9 (39.1)
	6–8 h	40	34 (85.0)	14 (38.9)
Total	0–8 h	162	113 (69.8)	45 (39.8)
Control IVM MII at 24 h		389	319 (82.0)	137 (42.9)

• *Significantly different at P < 0.01 compared with the other groups; ^awe defined embryos as good quality if they had at least six blastomeres, <20% anucleate fragments and no apparent morphologic abnormalities; PN, pronuclei.

Figure 1:

Bi-refringent spindles in living human oocytes imaged at maturation stage with the Polscope following IVM (A) MI oocyte had the spindle. (B) TI had the spindle located between cytoplasm and the PB. (C) MII oocyte had the spindle located under the first PB. Original magnification, ×200