桑葚期胚胎(morula)等級

培養滿4天96小時達桑葚期胚胎等級

A: 全融合 (最優)
B: 3/4融合 (優)
C: 1/2融合 (差)
D: <1/2融合 (最 差 )







http://humrep.oxfordjournals.org/content/17/6/1513.full

The neglected morula/compact stage embryo transfer

1. Jun Tao1,2,3, 
2. Robert Tamis1, 
3. Katharine Fink1, 
4. Brenda Williams2,
5. Tresa Nelson-White2 and 
6. Randall Craig2

+Author Affiliations

1. ¹Arizona Institute of Reproductive Medicine, Phoenix, Arizona and
2. ²Fertility Treatment Centre, Chandler, Arizona, USA

• Received April 4, 2001.
• Revision received November 26, 2001.
• Accepted January 29, 2002.

 

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Abstract

BACKGROUND: This retrospective study analysed the outcomes of 339 embryo transfers on either day 3 (n = 97) or day 4 (n = 242), and proposed a grading system for morula/compact embryos. METHODS: The morula/compact embryo grading was based on: (i) the proportion of blastomeres undergoing the compaction process; (ii) the morphology of the compacted multicellular mass; (iii) the embryo quality on day 2 and 3; and (iv) the amount of fragmentation. Embryo transfers were classified into groups as follows: group I: transferred with zero `good' embryos; group II: one `good' embryo; group III: two or more `good' embryos. RESULTS: Patients on day 4 were transferred with significantly fewer embryos in groups II and III (2.58 ± 0.9 and 2.35 ± 0.6 respectively) when compared with the correspondent day 3 transfers (3.81 ± 1.4 and 4.07 ± 0.9 respectively) (P < 0.05), but had the same or higher implantation and pregnancy rates. Analysing the patients who had transfers with all `good' embryos, day 4 transfer achieved a significantly higher implantation rate compared with day 3 transfer (46.4 versus 21.4%, P < 0.01), but the number of embryos transferred on day 4 was significantly lower than day 3 (2.1 ± 0.5 versus 3.5 ± 0.9, P < 0.01). CONCLUSIONS: The morula/compact embryos had great value for embryo selection, which significantly reduced the number of embryos needed for transfer.

Figure 1.

Photomicrographs of morula/compact embryos with different scores. (A) Grade 4 embryo. The embryo is nearly fully compacted with all blastomeres undergoing the compact process. Cell boundary is not clear but some nuclei can be identified.(B) Grade 3 embryo. More than three-quarters of blastomeres undergo the compact process. The embryo is close to sphere shape with a smooth profile. (C) Grade 2 embryo. This embryo has an irregular morphology with a deep indentation on the surface.(D) Grade 1. Fewer than half of the blastomeres undergo the compaction process and form a small compacted cell mass. Fragments and non-compacted blastomeres can be identified. Original magnification: ×200.