polescopy觀察具有紡錘體之卵細胞有較高受孕率胚胎品質與懷孕率

使用polescopy觀察卵細胞顯示
polescopy觀察具有紡錘體之卵細胞擁有較高受孕率胚胎品質與懷孕率
高齡卵細胞較高比例出現不具紡錘體影像之卵細胞

http://humrep.oxfordjournals.org/content/16/7/1464.full

Table I.

Fertilization and embryo development of human oocytes with or without birefringent spindle. Values in parentheses are percentages

Cycles	Spindle	No. of oocytes	No. of oocytes with 2PN	No. and stages of viable embryos on day 3			Day 5 embryosa	Day 6 embryosb
				Totalc	4–7celld	8–11celld
aData from 63 cycles.
bData from 41 cycles.
cPercentage of number of oocytes with 2PN.
dPercentage of total number of viable embryos on day 3.
eNo. of blastocysts and morulae/no. of 2 PN oocytes (from 63 cycles on day 5 and 41 cycles on day 6. For more details, see experimental designs).
fghijkValues with different superscripts in the same column are different significantly (f,gP < 0.05; h,iP < 0.01; j,kP < 0.001).
PN = pronuclei. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination W.H. Wang1,2,4,  L. Meng1,2,  R.J. Hackett1,2 and  D.L. Keefe1,2,3 +Author Affiliations 1Division of Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, Women & Infants Hospital of Rhode Island, Brown University School of Medicine, Providence, RI, 2Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, New England Medical Center, Boston, MA and 3Marine Biological Laboratory, Woods Hole, MA, USA Received December 11, 2000. Accepted April 4, 2001.   Next Section Abstract BACKGROUND: Birefringent spindles imaged with the Polscope can predict fertilization rates after intracytoplasmic sperm injection (ICSI). The present study examined the development of human oocytes with or without birefringent spindles, imaged with the Polscope before ICSI. METHODS: Oocytes were obtained from stimulated ovaries of consenting patients undergoing oocyte retrieval for ICSI. Spindles were imaged with the Polscope combined with a computerized image analysis system. After imaging and ICSI, oocytes with or without spindles were cultured separately for examination of fertilization and embryo development. A total of 1544 oocytes from 136 cycles were examined with the Polscope and inseminated by ICSI. RESULTS: Spindles were imaged in 82% of oocytes. After ICSI, more oocytes (P < 0.05) with spindles (69.4%) fertilized normally, forming 2 pronuclei, than oocytes without spindles (62.9%). At day 3, more oocytes (P < 0.01) with spindles (66.3%) developed to 4–11 cell stages than oocytes without spindles (55.4%). Significantly more (P < 0.001) oocytes with spindles developed to morula and blastocyst by day 5 (51.1 versus 30.3%) and day 6 (53.2 versus 29.3%) compared with oocytes without spindles. CONCLUSIONS: The results indicate that the presence of a birefringent spindle in human oocytes can predict not only higher fertilization rate, but also higher embryo developmental competence.