玻璃化冷凍解凍: 對胚胎中空透明帶外觀結構無明顯影響，對胚胎懷孕率著床率亦無明顯差異‧http://www.reproduction-online.org/content/139/1/121.full
A comparative analysis of the zona pellucida birefringence of fresh and frozen–thawed human embryos
+Author Affiliations
- Correspondence should be addressed to G-X Lu at Institute of Reproductive and Stem Cell Engineering, Central South University; Email:lugxdirector@yahoo.com.cn
Abstract
The cryopreservation of human embryos is thought to induce alteration in the glycoprotein matrix and lead to zona change. However, this assumption has been full of controversies till now. The objective of this study was to evaluate the effect of cryopreservation on zona pellucida of human embryos. Fresh (n=106, from 40 patients) and frozen–thawed embryos (n=123, from 40 patients) were obtained from consenting patients who received conventional IVF and ICSI treatment. The birefringence of zona pellucida in human fresh and frozen–thawed embryos was imaged and quantitatively analyzed using polarized light microscopy before embryo transfer. There was no significant difference in retardance and thickness of the zona pellucida multilaminar structure between the two groups. Pregnancy and implantation rates of transferred fresh and frozen–thawed embryos were also compared. No significant difference was found in the rates of clinical pregnancy (47.5 vs 37.5%) and implantation (24.5 vs 23.2%) between the two groups. This study suggests that there is no significant change in the zona pellucida birefringence of human embryos before and after cryopreservation.
Figure 1
The birefringent zona pellucida of human embryos. (A) Zona pellucida of human embryos is inconspicuously imaged by conventional Hoffmann inverted microscope; (B) birefringent zona pellucida of human embryos can be imaged distinctly using polarized light microscopy, which is multilaminar and displays obvious variance between embryos; birefringent zona pellucida of fresh (C) and frozen–thawed (D) human embryos was analyzed using polarized light microscopy before embryo transfer. Scale bar=10 μm.
Table 2
Comparison of the characteristics and clinical outcomes of patients undergoing fresh or frozen embryo transfer cycles.
| Fresh cycles | Frozen cycles | P value | |
|---|---|---|---|
| No. of patients | 40 | 40 | – |
| Age (years) | 29.8±4.1 | 29.1±3.5 | 0.419 |
| Type of infertility (%) | 0.179 | ||
| Primary | 16 (40.0) | 22 (55.0) | |
| Secondary | 24 (60.0) | 18 (45.0) | |
| Cause of infertility (%) | 0.612 | ||
| Tubal | 29 (72.5) | 26 (65.0) | |
| Male | 4 (10.0) | 7 (17.5) | |
| Mixed | 7 (17.5) | 7 (17.5) | |
| Insemination procedure (%) | 0.446 | ||
| IVF | 31 (77.5) | 28 (70) | |
| ICSI | 9 (22.5) | 12 (30) | |
| Clinical outcomes (%) | |||
| Clinical pregnancy | 19/40 (47.5) | 15/40 (37.5) | 0.366 |
| Implantation | 26/106 (24.5) | 19/82 (23.2) | 0.829 |
沒有留言:
張貼留言