2PN原核 vs. 2PB極體之相對位置與胚胎品質有關

2PB之間之距離與胚胎品質無關

2PN vs. 2PB之位置相對關聯性與ICSI 或IVF無關


2PN vs. 2PB之相對位置與胚胎品質有關
beta 角度越大，ICSI胚胎品質越差


http://humrep.oxfordjournals.org/content/14/10/2588.full

Pronuclear orientation, polar body placement, and embryo quality after intracytoplasmic sperm injection and in-vitro fertilization: further evidence for polarity in human oocytes?

1. C. Garello1,2,3, 
2. H. Baker1, 
3. J. Rai1, 
4. S. Montgomery1, 
5. P. Wilson1,
6. C.R. Kennedy1 and 
7. G.M. Hartshorne1,2,4

+Author Affiliations

1. ¹Centre for Reproductive Medicine, Walsgrave Hospital, Coventry, CV2 2DX and
2. ²Sir Quinton Hazell Molecular Medicine Research Centre, Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

• Received May 6, 1999.
• Accepted July 6, 1999.

 

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Abstract

Three hypotheses were tested: (i) the distance between first and second polar bodies (PB) may relate to embryo morphology, (ii) that the orientation of pronuclei (PN) relative to PB may relate to embryo morphology, (iii) that the placement of a spermatozoon in a fixed plane relative to the first PB [intracytoplasmic sperm injection (ICSI)] may alter PN/PB orientation relative to in-vitro fertilization (IVF). A total of 251 two pronuclear (2PN) embryos (124 ICSI, 127 IVF) from 64 patients was studied. Angles were measured between the PN axis and the nearest PB (α), the furthest PB (β), and between the two PB (γ). On day 2, the morphological grades of embryos were recorded. γ ranged from 0 to 150° and was not significantly different for ICSI or IVF embryos of different grades; however, an unusual distribution of γ suggested different populations of oocytes. The first hypothesis was rejected. α and β ranged from 0 to 90°: α did not relate significantly to embryo grade, but β increased significantly with decreasing quality of ICSI embryos (P < 0.05) and the total group (P < 0.01), supporting hypothesis (ii). The difference in β between ICSI and IVF embryos was not significant, so hypothesis (iii) was unproven. Significant differences between ICSI and IVF embryos in PN positions, irregular cleavage, and cleavage failure were noted.

Figure 1.

Diagram showing the measurement of α, β, and γ. α is the smallest angle between a line drawn through the axis of the pronuclei and the closest polar body. β is the smallest angle between the same line and the furthest polar body. γ is the angle between the two polar bodies using the centre of the oocyte for reference. The placement of these angles may appear differently depending upon the angle of observation and the orientation of the polar bodies.