品質較佳精蟲頭部polescope折射率較高

使用polescope觀察sperm head之折射率可用於挑選精蟲
品質較佳精蟲頭部折射率較高

http://www.ncbi.nlm.nih.gov/pubmed/18023436?dopt=Abstract

Fertil Steril. 2008 Jul;90(1):104-12. Epub 2007 Nov 26.

Sperm head's birefringence: a new criterion for sperm selection.

Gianaroli L, Magli MC, Collodel G, Moretti E, Ferraretti AP, Baccetti B.

Source

Società Italiana Studi di Medicina della Reproduzione (S.I.S.M.E.R.), Bologna, Italy. luca.gianaroli@sismer.it <luca.gianaroli@sismer.it>

Abstract

OBJECTIVE:

To investigate the characteristics of birefringence in human sperm heads and apply polarization microscopy for sperm selection at intracytoplasmic sperm injection (ICSI).

DESIGN:

Prospective randomized study.

SETTING:

Reproductive Medicine Unit, Società Italiana Studi Medicina della Riproduzione, Bologna, Italy.

PATIENT(S):

A total of 112 male patients had birefringent sperm selected for ICSI (study group). The clinical outcome was compared with that obtained in 119 couples who underwent a conventional ICSI cycle (control group).

INTERVENTION(S):

The proportion of birefringent spermatozoa was evaluated before and after treatment in relation to the sperm sample quality. Embryo development and clinical outcome in the study group were compared with those in the controls.

MAIN OUTCOME MEASURE(S):

Proportion of birefringent sperm heads, rates of fertilization, cleavage, pregnancy, implantation, and ongoing implantation.

RESULT(S):

The proportion of birefringent spermatozoa was significantly higher in normospermic samples when compared with oligoasthenoteratospermic samples with no progressive motility and testicular sperm extraction samples. Although fertilization and cleavage rates did not differ between the study and control groups, in the most severe male factor condition (oligoasthenoteratospermic with no progressive motility and testicular sperm extraction), the rates of clinical pregnancy, ongoing pregnancy, and implantation were significantly higher in the study group versus the controls.

CONCLUSION(S):

The analysis of birefringence in the sperm head could represent both a diagnostic tool and a novel method for sperm selection.