精蟲處理方法比較

上游法可取得數量較少，速度較快, 活動力比率較高, 形態正常比率較高之精蟲
分層離心法可取得較多精蟲 但速度較慢，形態品質較差
清洗法可取得最多精蟲，但品質好壞並無分離
精蟲處理方法之選擇必須視病人精蟲品質決定

http://www.ncbi.nlm.nih.gov/pubmed/1315792


Hum Reprod. 1992 Feb;7(2):261-6.

Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples.

Ng FL, Liu DY, Baker HW.

Source

University of Melbourne Department of Obstetrics and Gynaecology, Australia.

Abstract

Two methods of density gradient centrifugation, Percoll (P) and mini-Percoll (MP), were compared with the swim-up technique for preparing spermatozoa from each of 40 abnormal semen samples. P and MP produced similar results with a mean recovery of spermatozoa with progressive motility which was significantly higher (18-19%) than that achieved with swim-up (5%). However, the swim-up method resulted in the recovery of spermatozoa with a higher mean motility (89 versus 58%), velocity (69 versus 56 microns/s), percentage with normal morphology (22 versus 16%) and intact acrosomes (61 versus 36%) than P and MP. The mean amplitude of lateral head displacement was the only characteristic of spermatozoa in semen which correlated with the recoveries of motile spermatozoa. Combining MP and swim-up methods for 10 samples produced a higher recovery (11 versus 6.9%) of spermatozoa with significantly better mean motility (94 versus 87%) than did swim-up alone. Although P and MP resulted in greater yields of motile spermatozoa than the swim-up preparation, the latter procedure selected higher proportions of spermatozoa with improved characteristics (velocity, intact acrosomes and normal morphology) which correlate with fertilization rates in vitro. It is concluded that P and MP are not superior to swim-up. However, sequential MP and swim-up preparation improves yields of high quality spermatozoa from some abnormal semen samples and therefore has potential for improving fertilization rates.