培養液之白蛋白具去除毒素效果

培養液之白蛋白albumin(2 mg/ml )具減除toxin毒素效果
使用不含albumin之培養液可提高sperm motility assay之敏感性

20ml syringe對胚胎有相對毒性

http://humrep.oxfordjournals.org/content/15/7/1586.long

Table III.

Precision of the sperm motility assay conducted in the absence of albumin. Values are the mean SMI ± SD of three determinations conducted over 2–48 h incubation time

Item	2 h	4 h	6 h	8 h	24 h	48 h
Bold text represents sperm toxic items with a SMI <0.75. Table I. Sperm motility index (SMI) values after 2–48 h exposure to items 1–15, compared to the mouse embryo development (MED) assay Items SMI values in the presence of albumin at time (h) SMI values in the absence of albumin at time (h) MED after 72 h 2 4 6 8 24 48 2 4 6 8 24 48 Wilcoxon signed ranks test showed a significant treatment effect (P < 0.01), taking all 15 items into account and comparing albumin supplementation against absence of albumin, on the SMI values after 4, 8 and 48 h incubation periods. Bold text highlights results with a SMI <0.75 (sperm toxic) and MED <70% (mouse embryo toxic). aIndicates interassay variation of the procedure. 1. Monovette serum gel tube 0.59 0.32 0.08 0.08 0 0 0.05 0 0 0 0 0 32 2. Gelman Acrocap filter, 1st ml 0.97 0.97 0.97 1.00 0.91 0.66 1.03 0.89 0.89 0.63 0.03 0 78 3. Sartorius Sartolab P filter 1st ml 1.05 0.95 1.00 0.95 0.88 0.62 1.03 0.92 0.82 0.60 0.17 0 19 4. Primo syringe (20 ml) 1.07 0.95 0.95 1.03 0.83 1.03 1.02 0.99 1.00 0.87 0.54 0.61 89 5. Braun syringe (20 ml) 1.07 0.95 0.84 1.00 0.86 0.75 1.05 0.97 1.00 0.92 0.80 0.58 90 6. Terumo syringe (20 ml) 1.07 1.00 1.04 1.03 0.97 0.97 1.07 0.97 0.89 0.89 0.77 0.39 82 7. Glass pasteur pipettes (acid washed) 1.07 1.02 1.00 0.95 0.89 0.76 1.07 1.02 0.95 0.97 0.94 0.93 93 8. Gelman Acrocap filter, 10th ml 1.03 0.97 1.00 0.97 0.91 0.84 1.00 0.97 0.95 0.97 0.94 1.06 93 9. Millipore Sterivex filter, 1st ml 1.00 0.97 0.95 0.95 0.91 1.12 0.95 0.89 0.95 0.92 0.86 0.90 79 10. Millipore Sterivex filter, 10th ml 1.00 0.97 0.95 0.95 0.97 1.12 1.00 0.92 0.95 0.89 0.91 0.90 94 11. Sartorius Sartolab P filter, 10th ml 1.05 0.97 0.92 0.92 0.97 1.06 1.03 0.84 0.89 0.82 0.83 0.77 77 12. Falcon 2037 tissue culture tube 1.05 0.97 1.00 1.03 0.86 0.81 1.05 1.00 1.00 0.97 0.89 0.77 90 13. Falcon 2095 tissue culture tube 1.05 1.00 1.00 1.03 0.77 0.87 1.07 0.99 1.00 1.00 1.03 0.93 92 14. Falcon 2001 tissue culture tube 1.07 1.04 1.00 1.00 0.97 0.91 1.07 1.04 1.00 0.97 1.03 0.87 92 15. Falcon 2003 tissue culture tube 1.07 1.04 1.00 1.03 0.91 1.00 1.07 1.04 1.00 1.00 0.97 0.97 90 Average relative deviation of replicate determinationsa (%) 9.6 1.0 0.5 7.6 4.1 4.1 1.9 3.1 0 5.1 0.2 15.2 <10
Inter-assay (between day) variation
12. Falcon 2037 tissue culture tube	0.99 ± 0.01	0.99 ± 0.01	0.99 ± 0.02	0.98 ± 0.02	0.91 ± 0.13	0.50 ± 0.12
20. Vacutainer SST tube	0.80 ± 0.08	0.53 ± 0.16	0.30 ± 0.23	0.14 ± 0.21	0.00 ± 0.00	0.00 ± 0.00
Intra-assay (within day) variation
12. Falcon 2037 tissue culture tube	0.99 ± 0.02	1.00 ± 0.00	0.97 ± 0.02	0.96 ± 0.00	0.91 ± 0.03	0.48 ± 0.22
20. Vacutainer SST tube	0.77 ± 0.06	0.47 ± 0.23	0.29 ± 0.03	0.03 ± 0.03	0.00 ± 0.00	0.00 ± 0.00