顯示具有 ICSI 標籤的文章。顯示所有文章

2015年10月10日

卵子受孕方式會影響早期胚胎之分裂速度

卵子受孕方式(IVF vs ICSI)會影響早期胚胎之分裂速度
IVF速度慢於ICSI約1.5h

晚期胚胎影響較不明顯
可見晚期胚胎分裂速度主要取決於胚胎之品質

http://www.ncbi.nlm.nih.gov/pubmed/26307686

2014年6月5日

IVF後6h未受孕卵施行rescue ICSI

IVF後6h 檢查PB以確定是否受孕
未受孕卵施行rescue ICSI, 懷孕率12%

http://www.ncbi.nlm.nih.gov/pubmed/16580372/

2014年6月2日

多餘卵子捐贈後施行ICSI-ET，懷孕率達47%

取卵超過20顆卵子可將多餘卵子冷凍並不明顯影響PR

多餘卵子捐贈後施行ICSI-ET，懷孕率達47%

http://www.ncbi.nlm.nih.gov/pubmed/12513887/

2014年4月19日

ICSI未受孕主因: 卵細胞未被激活

ICSI後卵子未受孕主因: 卵細胞未被激活

http://www.ncbi.nlm.nih.gov/pubmed/9663780

2013年9月18日

副睪取精+ICSI可得50%優質胚胎

副睪取精PESA+ICSI可得50%優質胚胎

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800539/

2013年9月14日

Endometriosis 病患prefer ICSI

Endometriosis IVF病患可能較適合ICSI insemination

http://www.ncbi.nlm.nih.gov/pubmed/23271211

2013年6月25日

不明原因不孕可考慮ICSI避免卵子全無受精

不明原因不孕症之病患必要時須考慮精蟲顯微注射授精ICSI方式以避免卵子全無受精

http://www.sciencedirect.com/science/article/pii/S0015028213005529

2013年6月13日

IVM卵子以ICSI或IVF授精並無明顯差異

體外不成熟卵子經培養IVM後，再以ICSI或IVF授精後，胚胎受孕率或著床率並無明顯差異

http://www.ncbi.nlm.nih.gov/pubmed/23063820

2012 Dec;25(6):603-7. doi: 10.1016/j.rbmo.2012.08.001. Epub 2012 Aug 31.

IVF versus ICSI for the fertilization of in-vitro matured human oocytes.

Walls M, Junk S, Ryan JP, Hart R.

Source

Fertility Specialists WA, 25 Queenslea Drive, Claremont, Perth, Western Australia 6010, Australia. melanie@fertilitywa.com.au

Abstract

Traditional dogma suggests that intracytoplasmic sperm injection (ICSI) should be performed to ensure successful oocyte fertilization in an in-vitro maturation (IVM) cycle. This study postulated that there would be no difference in the fertilization rate when ICSI was compared with IVF. This hypothesis was tested in a randomized trial of IVF versus ICSI in IVM. A total of 150 immature oocytes were collected in eight cycles of IVM for patients diagnosed with polycystic ovarian syndrome (PCOS). Patients were primed with minimal FSH before transvaginal oocyte aspiration. Sibling oocytes were inseminated by 50% IVF and 50% ICSI. There was no significant difference in fertilization, useable or total blastocyst development between the two insemination technique groups. Clinical pregnancy results for combined fresh and cryopreserved transfers were identical between the two insemination techniques with a total of two fresh and five cryopreserved IVF-inseminated embryos resulting in three clinical pregnancies (42.9%) and five fresh and two cryopreserved ICSI-derived embryos resulting in three clinical pregnancies (42.9%). This research has shown IVF to be a legitimate fertilization technique for IVM oocytes in PCOS patients and provides a greater awareness of the use of a fertilization method previously not utilized with IVM. In-vitro maturation (IVM) is an alternative treatment method to traditional IVF. Due to the minimal use of stimulating hormones in this treatment, IVM has a lower risk of ovarian hyperstimulation syndrome, it can be used for fertility preservation in cancer patients and it is more cost conservative. Early research into the effects of IVM showed a hardening effect on the membrane surrounding the egg (the zona pellucida). It was initially believed that, to overcome this hardening in order to allow the egg to be fertilized, spermatozoa would need to be injected into the egg using intracytoplasmic sperm injection. Due to recent advances in hormonal stimulation protocols (FSH priming) and culture conditions, we postulated that, for patients suffering from polycystic ovarian syndrome (PCOS), fertilization, embryo development and clinical pregnancy would not be superior in the injected oocytes compared with those inseminated by IVF. We found that by using the two insemination techniques on sibling oocytes from eight PCOS patients, there was no significant difference in fertilization, useable or total blastocyst development (day 5 or 6 embryos) and that clinical pregnancy results were identical. This research provides a greater awareness of a fertilization technique which is not normally utilized for IVM treatment, providing a less invasive, more cost-effective approach for the patient.

2013年5月6日

不同受孕方式會影響胚胎孵化方式

以time-lapse培養系統觀察胚胎孵化之方式，
發現不同受孕方式(IVF 或 ICSI)會影響囊胚期胚胎孵化之方式

ICSI好發生type 1 孵化
IVF 好發生type 2 孵化

Type 1 was characterized by penetration of the zona pellucida (ZP) by small trophectoderm projections,
type 2 was preceded by a regular rupture of the ZP followed by extrusion of the blastocyst.

http://www.ncbi.nlm.nih.gov/pubmed/23909991