卵子冷凍解凍存活率約80%

卵子冷凍解凍存活率約80%
Open system 仍是胚胎玻璃化冷凍之主流

http://humrep.oxfordjournals.org/content/25/5/1192.full

Embryo development and gestation using fresh and vitrified oocytes

1. Carlos Gilberto Almodin*, 
2. Vania Cibele Minguetti-Camara,
3. Cassia Lopes Paixao and 
4. Priscila Cardoso Pereira

+Author Affiliations

1. Materbaby Human Reproduction - Human Reproduction, Av XV de Novembro, 1232, Maringá, Paraná 87013230, Brazil

1. *Correspondence address. Tel: +55-44-32243992; Fax: +55-44-32251162; E-mail:almodin@materbaby.com.br

• Received September 16, 2009.
• Revision received January 18, 2010.
• Accepted January 28, 2010.

 

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Abstract

BACKGROUND The objective of this study was to compare the gestational results obtained with vitrified/thawed oocytes by a novel vitrification method (Vitri-ingá) to results obtained with fresh oocytes.

METHODS A total of 125 IVF-ET procedures carried out over 2008 were analysed, in which 79 patients received embryos from fresh oocytes (Group 1), and 46 patients received embryos from vitrified/thawed oocytes using Vitri-ingá (Group 2). Fresh and vitrified/thawed oocytes were fertilized and embryos were transferred. Fertilization, pregnancy and implantation rates were compared.

RESULTS Vitrified oocytes presented a survival rate of 84.9%. Fertilization, pregnancy and implantation rates showed no statistically significant differences between fresh and cryopreserved/thawed groups (81.3, 51.9, 21.3% and 80.8, 45.6 and 14.9%, respectively). Only the average number of blastomeres in Group 1 (6.86 ± 2.07) was significantly higher than in Group 2 (6.35 ± 2.26; P = 0.010).

CONCLUSIONS Despite some differences in the patient groups, the clinical results of this study demonstrated that the Vitri-ingá method preserves the potential of human vitrified oocytes for fertilization and further development.

Table II

Fertilization, pregnancy and implantation rates of fresh and vitrified/thawed human oocytes.

	Fresh oocytes (Group 1)	Vitrified/thawed oocytes (Group 2)	P
No. of cycles	79	46
Patients age*	34.2 ± 4.5	35.4 ± 5.0	0.191
No. of fresh oocytes	906	–
No. of thawed oocytes	–	252
No. of surviving thawed oocytes (%)	–	214 (84.9%)
No. microinjected oocytes	413	214
No. of fertilized oocytes (%)	336 (81.3%)	173 (80.8%)	0.876
No. of transferred embryos*	3.57 ± 1.03	3.46 ± 1.34	0.318
No. of blastomeres*	6.86 ± 2.07	6.35 ± 2.26	0.010**
Total of grade I and II embryos (%)	158 (47.0%)	69 (39.9%)	0.125
Pregnancy rate per transfer (%)	41 (51.9%)	21 (45.6%)	0.500
Implantation rate (%)	21.3%	14.9%	0.089

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