cryoloop冷凍囊胚植入懷孕率約37%

使用cryoloop冷凍解凍囊胚期胚胎存活率約80%
植入懷孕率約37%
第5天囊胚之冷凍解凍存活率較第6天囊胚為高
流產率約20%


http://humrep.oxfordjournals.org/content/18/2/384.full

Vitrification of human blastocysts using cryoloops: clinical outcome of 223 cycles*

1. T. Mukaida1,5, 
2. S. Nakamura1, 
3. T. Tomiyama2, 
4. S. Wada2, 
5. C. Oka3, 
6. M. Kasai4and 
7. K. Takahashi1

+Author Affiliations

1. ¹Hiroshima HART Clinic, 5‐7‐10 Ohtemchi, Naka‐ku, Hiroshima,
2. ²Osaka HART Clinic, Snowcrystal 10F 2‐6‐20, Umeda, Kita‐ku, Osaka,
3. ³Tokyo HART Clinic, 1‐22‐2, Higashi, Shibuya‐ku, Tokyo and
4. ⁴Laboratory of Animal Science, College of Agriculture, Kochi University, Nankoku, Kochi, Japan

1. 5To whom correspondence should be addressed at: Hiroshima HART Clinic, 5‐7‐10 Ohtemchi, Naka‐ku, Hiroshima, 730‐0051 Japan. e‐mail: hart@enjoy.ne.jp

• Received August 27, 2002.
• Accepted October 8, 2002.

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Abstract

BACKGROUND: The need to cryopreserve human blastocysts is increasing. The successful birth has been reported of a baby from a blastocyst vitrified using the cryoloop technique. The present study expands on this earlier report to confirm the effectiveness of this vitrification procedure. METHODS: In patients undergoing IVF at one of three clinics, supernumerary blastocysts on day 5 or 6 at various stages of development were vitrified using cryoloops. RESULTS: Of 725 vitrified blastocysts, 583 (80.4%) survived. After the transfer of 493 blastocysts in 207 cycles, 76 women (37%) became clinically pregnant. Among these women, 21 pregnancies ended in miscarriage, 23 healthy babies were born in 18 deliveries, and 37 pregnancies are ongoing. The survival rate of day 5 blastocysts (87%) was higher than that of day 6 blastocysts (55%), but implantation rates and pregnancy rates were not statistically significantly different. CONCLUSIONS: Clinical outcomes with 725 blastocysts and 207 transfers showed that vitrification using cryoloops is effective and practical for the cryopreservation of human blastocysts. Early blastocysts on day 5 seem to be the most suitable in terms of stage and age for cryopreservation, but developed and day 6 blastocysts can also be cryopreserved.

Table III.

Pregnancy rates after transfer of vitrified blastocysts classified by the most developed stage of the group of blastocysts of each patient before vitrification

Groups classified by development*	No. of pregnancies/no. of transfers (%)
	Day 5	Day 6	Total
I	8/19 (42)	‐	8/19 (42)
II	9/19 (47)	‐	9/19 (47)
III	18/42 (43)	2/5 (40)	20/47 (43)
IV	22/65 (34)	4/18 (22)	26/83 (31)
V	7/20 (35)	6/19 (32)	13/39 (33)
Total	64/165 (39)	12/42 (29)	76/207 (37)

^*Embryos were classified as defined in Table II.

No significant difference was found among the rates in the same column.