冷凍保存成功率 (高到低): Day5, Day1, Day3, Day2胚胎

不成熟卵子冷凍保存成功率較高

冷凍保存成功率: Day5>Day1>Day3>Day2胚胎

http://www.reproduction-online.org/content/141/1/1.full


Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

1. Joseph Saragusty and 
 
2. Amir Arav1

+Author Affiliations

1. Department of Reproduction Management,Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, D-10315 Berlin, Germany
   ¹Institute of Animal Sciences,Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel

1. Correspondence should be addressed to A Arav; Email: arav@agri.huji.ac.il

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Abstract

Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two basic cryopreservation techniques rule the field – controlled-rate freezing, the first to be developed, and vitrification, which, in recent years, has gained a foothold. While much progress has been achieved in human medicine, the cattle industry, and in laboratory animals, this is far from being the case for most other mammals and even less so for other vertebrates. The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.

Figure 1

Vitrification surface carrier systems: (1) electron microscope grid, (2) minimum drop size, (3)* Cryotop, (4)* Cryoloop, (5) Hemi-straw, (6)* Cryoleaf, (7)* fiber plug, (8)* direct cover vitrification, (9)* vitrification spatula, (10) nylon mesh; arrow points at the nylon mesh, (11)* plastic blade, (12)* Vitri-Inga. *These photos were kindly provided by Masa Kuwayama (3), Juergen Liebermann (4, 7), Ri-Cheng Chian (6), Shee-Uan Chen (8), King L Chow and Wai Hung Tsang (9), Koji Nakagawa (11), and Carlos Gilberto Almodin (12). Picture reprinted with minor revisions fromMatsumoto H, Jiang JY, Tanaka T, Sasada H & Sato E 2001 Vitrification of large quantities of immature bovine oocytes using nylon mesh. Cryobiology 42 139–144, with permission from Elsevier. © 2001 Elsevier.

Figure 2

Vitrification tubing carrier systems: (1 top)* plastic straw, (1, 2nd from top)* open-pulled straw, (1, 3rd from top)* superfine open-pulled straw, (1 bottom)* flexipet-denuding pipette, (2)* CryoTip, (3)* high-security vitrification device, (4)* pipette tip, (5) sealed pulled straw, (6)* Cryopette, (7)* Rapid-i, and (8)* JY Straw. *These photos were kindly provided by Juergen Liebermann (1, 3, 6, 7), Masa Kuwayama (2), John Engelhardt (4), and Ri-Cheng Chian (8).