卵子捐贈可冷凍日後解凍而達到與新鮮卵子類似懷孕率

卵子捐贈可先冷凍，日後解凍後受孕植入
可達到與新鮮卵子類似懷孕率

如此不需調控卵子捐贈者與受贈者之月經周期即可執行試管植入

http://humrep.oxfordjournals.org/content/26/4/782.full

Efficacy of oocyte vitrification combined with blastocyst stage transfer in an egg donation program

1. Javier I. García*, 
2. Luis Noriega-Portella and 
3. Luis Noriega-Hoces

+Author Affiliations

1. Laboratory of Assisted Reproduction, PRANOR Group of Assisted Reproduction, Concebir Clinic, Los Olivos 364, San Isidro, Lima, Peru

1. *Correspondence address. Tel: +51-1-422-5246; E-mail: jgarciaf@hotmail.com

• Received May 18, 2010.
• Revision received November 29, 2010.
• Accepted January 7, 2011.

 

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Abstract

BACKGROUND A successful oocyte vitrification program is important for women with various indications for assisted reproduction technology. The objective of this study was to report the outcome of vitrification of oocytes, obtained through an oocyte donation program, by evaluating the embryo development, pregnancy and implantation rates (IRs) after blastocyst transfer.

METHODS A total of 1098 oocytes were obtained from 78 donors. There were 312 oocytes used in the study group (vitrified oocytes) and 786 used in the control group (fresh oocytes). There were 34 recipients who received blastocysts obtained from vitrified oocytes and 58 recipients who received blastocysts from fresh oocytes. The fertilization rate, cleavage rate, embryo quality, pregnancy rate (PR) and IR were compared between groups.

RESULTS Vitrified oocytes showed a survival rate of 89.4%. There was no difference in the fertilization rate (76.1 and 87.5%), Day 2 cleavage rate (96.3 and 98.0%) or blastocyst formation rate (41.3 and 45.3%) for the study and control groups, respectively. PRs, IRs and miscarriages rates (MRs) were similar for the study group compared with the control group (PR: 61.8 versus 60.0%; IR: 43.9 versus 42.9%; MR: 9.5 versus 5.9%).

CONCLUSIONS The developmental competence of embryos obtained from vitrified oocytes is not affected by the vitrification procedure, since they preserve the potential to be fertilized and to develop into high-quality blastocysts, similar to embryos from fresh oocytes. The successful clinical outcome indicates the use of this procedure for oocyte donation programs and for oocyte storage in general.

Table I

Comparison of laboratory results between both the study and the control group.

	Study group	Control group	P
No. total oocytes	312	786
No. total MII oocytes	283	696
No. total vitrified oocytes	283
No. total oocytes survival (%)	253 (89.4)
No. total injected oocytes	251	695
No. total fertilized oocytes (2PN) (%)	191 (76.1)	608 (87.5)
Cleavage rate by embryo at Day 2 (%)	96.3	98.0	0.883
No. cell/embryo at Day 2 (Mean ± SD)	3.69 ± 0.73	3.69 ± 0.85	1.000
Good-quality embryos at Day 2 (%)	90.8	84.2	0.543
No. cell/embryo at Day 3 (Mean ± SD)	7.39 ± 0.75	7.39 ± 1.11	1.000
Good-quality embryos at day 3 (%)	90.0	82.2	0.466
No. total morulae at Day 4 (%)	121 (63.4)	361 (59.4)
No. total blastocyst (%)	79 (41.3)	276 (45.3)
Good-quality blastocysts (%)	75.0	77.4	0.873
Early blastocyst (%)	25	18.9	0.607
Full blastocyst (%)	36.8	23.3	0.079
Expanded blastocyst (%)	35.5	52.6	0.111
Hatching blastocyst (%)	2.6	1.5	0.506

Table II

Clinical outcomes in the study and the control group.

	Study group	Control group	P
Recipients	34	85
No. of warmed oocyte/recipient (Mean ± SD)	7.44 ± 2.08
No. of injected oocytes/recipient (Mean ± SD)	7.38 ± 2.07	8.18 ± 3.03	0.080
No. of fertilized oocytes/recipient (Mean ± SD)	5.62 ± 1.94	7.15 ± 2.87	0.530
No. of blastocyst/recipient (Mean ± SD)	2.45 ± 1.23a	3.33 ± 1.93	0.008
No. total blastocyst transferred	66 (1.94 ± 0.24)	168 (1.98 ± 0.15)	0.138
Pregnancy rate (%)	61.8	60.0	0.930
Implantation rate (%)	43.9	42.9	0.925
Single pregnancy (%)	61.9	58.8	0.904
Twin pregnancy (%)	38.1	41.2	0.874
Miscarriages (%)	9.5	5.9	0.609
Biochemical pregnancy rate (%)	4.6	3.8	0.882

• Note: Values in parentheses are mean blastocyst transferred/recipient.
• ^aP < 0.05 compared to the control group.