添加glycine 1mM於傳統玻璃化冷凍解凍液中，可提高鼠卵GV冷凍解凍存活率
添加glycine 1mM於傳統IVM培養液中，可提高鼠不成熟卵GV成熟率

Sci Rep. 2016 Nov 15;6:37262. doi: 10.1038/srep37262.

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage.

Cao XY1,2, Rose J3, Wang SY1,2, Liu Y4, Zhao M1,2, Xing MJ1,2, Chang T1,2, Xu B1,2.

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Abstract

Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse germinal vesicle stage oocyte and/or subsequent maturation on its development. The data showed that glycine supplementation in either vitrification/thawing or maturation medium significantly improved the cytoplasmic maturation of MII oocytes manifested by spindle assembly, chromosomal alignment, mitochondrial distribution, euploidy rate, and blastocyst development following fertilization in vitro, compared to the control without glycine treatment. Furthermore, glycine addition during both vitrification/thawing and maturation further enhanced the oocyte quality demonstrated by various markers, including ATP contents and embryo development. Lastly, the effect of anti-apoptosis was also observed when glycine was added during vitrification. Our result suggests that reducing osmotic stress induced by vitrification could improve the development of vitrified mouse oocyte.