約2/3(67%)不成熟卵子(MI, GV)可經由體外培養形成成熟卵子(MII)
這些卵子經由ICSI受孕率約50%，受孕後進一步分裂成胚胎約30%

Arch Gynecol Obstet. 2015 Aug;292(2):459-63. doi: 10.1007/s00404-015-3644-y. Epub 2015 Feb 12.

Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development.

Ashourzadeh S1, Khalili MA, Omidi M, Mahani SN, Kalantar SM, Aflatoonian A, Habibzadeh V.

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Abstract

PURPOSE:

Recently, the upgrading of in vitro maturation (IVM) of human oocytes as a promising strategy has emerged in assisted reproductive technology (ART). The goal was to evaluate the correlation of the in vitro matured oocytes selected on the basis of the zona pellucida (ZP) birefringence and meiotic spindles (MS) detection with fertilization and subsequent embryo development in ICSIprogram.

METHODS:

A total of 168 immature oocytes [germinal vesicle (n = 140) and metaphase I (n = 28)] obtained from patients undergoing oocytes retrieval for ICSI. After in vitro culture for 24-40 h, 112 (67 %) oocytes reached to MII stage. Using a polarized microscopy, the presence of MS and ZP birefringence were assessed in matured oocytes, followed by ICSI performance.

RESULTS:

The rates of fertilization in oocytes with spindles (51.3 %) were similar to that of the oocytes without spindles (50.7 %; P = 1.00). Moreover, the fertilization rates in high birefringence (HB) oocytes was not statistically different than oocytes with low birefringence (LB) (P = 0.44). The findings also showed that 64.9 % of the fertilized oocytes developed to embryos, in which 33.3 % were derived from spindle-detected oocytes. Regarding the ZP birefringence, 35.5 % of the embryos were derived from HB oocytes.

CONCLUSIONS:

There were insignificant relationships between the MS detection and ZP birefringence score with the rates of fertilization and embryo development in IVM oocytes.