ICSI受孕率偏低(<50%)之病患，可採用培養液添加
Ca(2+)-ionophore solution (A23187)，
於ICSI後可刺激受精卵以增加卵子受孕率
Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study.
Ebner T1,
Montag M2;
Oocyte Activation Study Group,
Montag M2,
Van der Ven K2,
Van der Ven H2,
Ebner T3,
Shebl O3,
Oppelt P3,
Hirchenhain J4,
Krüssel J4,
Maxrath B5,
Gnoth C5,
Friol K5,
Tigges J5,
Wünsch E6,
Luckhaus J6,
Beerkotte A6,
Weiss D7,
Grunwald K7,
Struller D8,
Etien C8.
Abstract
Artificial oocyte activation has been proposed as a suitable means to overcome the problem of failed or impaired fertilization after intracytoplasmic sperm injection (ICSI). In a multicentre setting artificial oocyte activation was applied to 101 patients who were diagnosed with fertilization abnormalities (e.g. less than 50% fertilized oocytes) in a previous conventional ICSI cycle. Female gametes were activated for 15 min immediately after ICSI using a ready-to-use Ca(2+)-ionophore solution (A23187). Fertilization, pregnancy and live birth rates were compared with the preceding cycle without activation. The fertilization rate of 48% in the study cycles was significantly higher compared with the 25% in the control cycles (P < 0.001). Further splitting of the historical control group into failed (0%), low (1-30%) and moderate fertilization rate (31-50%) showed that all groups significantly benefitted (P < 0.001) in the ionophore cycle. Fewer patients had their embryo transfer cancelled compared with their previous treatments (1/101 versus 15/101). In total, 99% of the patients had an improved outcome with A23187 application resulting in a 28% live birth rate (35 babies). These data suggest that artificial oocyte activation using a ready-to-use compound is an efficient method.
Copyright © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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