胚胎紡錘絲之形成時間與胚胎碎片多寡有關

胚胎呈現正常之紡錘絲之形成時間(1st, 2nd, 3rd有絲分裂)其胚胎碎片較少

減數分裂紡錘絲出現於施打HCG後35.5h

1st有絲分裂(1>2cell): ICSI後28.2h

2nd有絲分裂(2>3cell): ICSI後38h

3rd有絲分裂(3>4cell): ICSI後39.1h

胚胎呈現不正常之紡錘絲之形成時間其胚胎碎片較多

減數分裂紡錘絲出現於施打HCG後36.5h

1st有絲分裂(1>2cell): ICSI後29.8h

2nd有絲分裂(2>3cell): ICSI後36.4h

3rd有絲分裂(3>4cell): ICSI後40.5h

Fertil Steril. 2015 Feb;103(2):374-81.e4. doi: 10.1016/j.fertnstert.2014.10.031. Epub 2014 Nov 20.

Fragmentation of human cleavage-stage embryos is related to the progression through meiotic and mitotic cell cycles.

Stensen MH1, Tanbo TG2, Storeng R3, Åbyholm T4, Fedorcsak P5.

Author information

Abstract

OBJECTIVE:

To study whether fragmentation of human embryos is related to the progression through meiotic and mitotic cell cycles.

DESIGN:

This report consists of two observational studies.

SETTING:

Not applicable.

PATIENT(S):

A total of 1,943 oocytes from 297 patients and 372 embryos from 100 patients were imaged in the Polscope instrument and monitored in the Embryoscope, respectively.

INTERVENTION(S):

Completion of the first meiotic division was determined by visualization of the meiotic metaphase II spindle in human oocytes, and the duration of the first three mitotic cell cycles was determined with time-lapse microscopy. The percentage of embryo fragmentation was recorded 42-45 hours after insemination.

MAIN OUTCOME MEASURE(S):

Appearance of the meiotic spindle; durations of the first, second, and third mitoses.

RESULT(S):

Human embryos with a low degree of fragmentation (<10%) at 42-45 hours after insemination originated from oocytes with an early appearance of the meiotic spindle (mean 35.5 hours after hCG injection), early first mitosis (28.2 hours after insemination), late start of the second mitosis (38.0 hours after insemination), and a shorter duration of the third mitosis (1.1 hours). Highly fragmented embryos (>50% fragmentation) originated from oocytes with a late-appearing meiotic spindle (36.5 hours after hCG injection), delayed initiation of the first mitosis (29.8 hours after insemination), early start of the second mitosis (36.4 hours after insemination), and a longer duration of the third mitotic cell cycle (4.1 hours).

CONCLUSION(S):

The observed associations suggest that the process of fragmentation of in vitro-derived embryos was related to the progress of the meiotic and the mitotic cell cycles.