卵子細胞膜無彈性於施行ICSI後卵子壞死機率較高(24%)受孕率較低(65%)
但胚胎形成囊胚機率無明顯差異(60-65%)，懷孕率無形顯差異(50%)

J Assist Reprod Genet. 2016 Sep 17. [Epub ahead of print]

Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection.

Mizobe Y1,2, Oya N3, Iwakiri R3, Yoshida N3, Sato Y3, Onoue N3, Miyoshi K4, Tokunaga M3, Ezono Y3.

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Abstract

PURPOSE:

In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.

METHODS:

Oocytes were divided into three groups-normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.

RESULTS:

The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7-67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).

CONCLUSIONS:

The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.