運用極體顯微鏡polscopy偵測卵子紡錘絲
成熟MII卵子約有92%可偵測到紡錘絲
偵測不到紡錘絲之卵子品質較差孕率較低

卵子的紡錘絲較大，較明顯，較靠近polar body--->卵子品質較佳&懷孕率較高




https://www.ncbi.nlm.nih.gov/pubmed/21882015#

J Assist Reprod Genet. 2011 Nov;28(11):1099-104. doi: 10.1007/s10815-011-9634-5. Epub 2011 Sep 1.

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection.

Tomari H1, Honjou K, Nagata Y, Horiuchi T.

Author information

Abstract

PURPOSE:

To investigate the relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection (ICSI).

METHODS:

Zygotes that had cleaved to two-cell stage by 27 h post-ICSI were classified as early cleaving and the remainder as late cleaving. Meiotic spindle parameters previously imaged using the PolScope were compared between the two groups.

RESULTS:

Of 384 embryos, 163 were classed as early cleaving and 221 as late cleaving. The rate of blastocyst formation or pregnancy by Day 2 embryo transfer was significantly higher following early cleavage than after late cleavage (52.4% vs. 24.4% or 32.6% vs. 11.4%). Spindle areas (108.0 vs. 89.8 μm(2)), lengths (14.7 vs. 13.4 μm) and PolScope retardance were also significantly greater in the early cleaving group.

CONCLUSIONS:

Meiotic spindle parameters determine the timing of the first zygotic cleavage and are strong indicators of human embryo developmental potential.