使用年輕去核捐贈卵子當受體，將受孕高齡病人之胚胎之原核PN取出後植入去核卵子中，形成胚胎植入後順利懷孕

Reprod Biomed Online. 2016 Oct;33(4):529-533. doi: 10.1016/j.rbmo.2016.07.008. Epub 2016 Aug 1.

Pregnancy derived from human zygote pronuclear transfer in a patient who had arrested embryos after IVF.

Zhang J1, Zhuang G2, Zeng Y2, Grifo J3, Acosta C2, Shu Y2, Liu H4.

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Abstract

Nuclear transfer of an oocyte into the cytoplasm of another enucleated oocyte has shown that embryogenesis and implantation are influenced by cytoplasmic factors. We report a case of a 30-year-old nulligravida woman who had two failed IVF cycles characterized by all her embryos arresting at the two-cell stage and ultimately had pronuclear transfer using donor oocytes. After her third IVF cycle, eight out of 12 patient oocytes and 12 out of 15 donor oocytes were fertilized. The patient's pronuclei were transferred subzonally into an enucleated donor cytoplasm resulting in seven reconstructed zygotes. Five viable reconstructed embryos were transferred into the patient's uterus resulting in a triplet pregnancy with fetal heartbeats, normal karyotypes and nuclear genetic fingerprinting matching the mother's genetic fingerprinting. Fetal mitochondrial DNA profiles were identical to those from donor cytoplasm with no detection of patient's mitochondrial DNA. This report suggests that a potentially viable pregnancy with normal karyotype can be achieved through pronuclear transfer. Ongoing work to establish the efficacy and safety of pronuclear transfer will result in its use as an aid for human reproduction.