單層80%細胞分層液 vs. 上游法
concentration (67 x 10(6) versus 42 x 10(6)/ml, P < 0.001),
motility (66 versus 59%, P < 0.001),
morphology (56 versus 49%, P < 0.005)
percentage recovery (60 versus 42%, P < 0.005).
Hum Reprod. 1995 Dec;10(12):3195-7.
motility (66 versus 59%, P < 0.001),
morphology (56 versus 49%, P < 0.005)
percentage recovery (60 versus 42%, P < 0.005).
Hum Reprod. 1995 Dec;10(12):3195-7.
A Mono-Percoll separation technique improves sperm recovery of normal and male factor specimens when compared with the swim-up technique.
Source
Fertility Center of Maryland, Towson 21204, USA.
Abstract
The purpose of this study was to determine the effects of a simplified 80% Mono-Percoll sperm separation procedure on both normal and male factor semen samples compared with the standard swim-up technique. The parameters examined include sperm concentration, motility and morphology, total motile functional spermatozoa and percentage recovery. Normal patients demonstrated enhanced sperm parameters with the Mono-Percoll compared with the swim-up technique for concentration (67 x 10(6) versus 42 x 10(6)/ml, P < 0.001), motility (66 versus 59%, P < 0.001), morphology (56 versus 49%, P < 0.005) and percentage recovery (60 versus 42%, P < 0.005). Male factor patients showed enhanced sperm parameters with the Mono-Percoll procedure compared with the swim-up technique for motility (53 versus 42%, P < 0.05) and percentage recovery (54 versus 29%, P < 0.005), with no significant difference in concentration and morphology. In summary, the Mono-Percoll sperm recovery procedure is significantly better than the swim-up technique for male factor patients and patients with normal sperm parameters.
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