胚胎極體切片對胚胎之品質有不良影響

胚胎極體(polar body)切片作為胚胎基因診斷(preimplantation genetic diagnosis)，

對第2-3天胚胎之品質與胚葉細胞分裂有不良影響

Fertil Steril. 2012 Feb 23. [Epub ahead of print]

Effects of laser polar-body biopsy on embryo quality.

Levin I, Almog B, Shwartz T, Gold V, Ben-Yosef D, Shaubi M, Amit A, Malcov M.

SourceSarah Racine IVF Unit, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

Abstract

OBJECTIVE: To evaluate the effect of laser polar-body biopsy (PBB) for preimplantation genetic diagnosis on embryo quality.

STUDY DESIGN: Retrospective case-control analysis. The quality of 145 embryos after PBB was compared to 276 embryos of the same group of women without biopsy.

SETTING: University-based tertiary-care medical center.

PATIENT(S): Women with inherited genetics disease.

INTERVENTION(S): Laser PBB of IVF embryos for genetic diagnosis.

MAIN OUTCOME MEASURE(S): The study and control embryos were compared for fertilization rate, pronuclear grading, and cleavage-stage parameters on days 1, 2, and 3 after oocyte retrieval.

RESULT(S): The study embryos demonstrated higher rates of cleavage arrest (3.6% vs. 0.7%), higher rate of significant fragmentation on day 2 (9.5% vs. 3.0%), and lower rate of good cleavage embryos on day 2 (69.1% vs. 78.4%) compared with control embryos. On day 3, the study embryos had lower cleavage rates (six or more blastomeres; 56.5% vs. 74.5%), higher fragmentation (11.7% vs. 3.9%), higher rate of embryos presenting inferior cleavage pattern (57.2% vs. 38.5%), and lower mean blastomere number (5.8 ± 2.1 vs.6.6 ± 1.9) compared with control embryos.

CONCLUSION(S): Polar-body biopsy may have a negative effect on embryo quality.