冷凍卵子須先去除卵丘細胞以提高抗凍液之效果

冷凍卵子須先去除卵丘細胞以提高抗凍液之效果

去除卵丘細胞 冷凍解凍後之卵子細胞，其紡錘絲可獲較高保存率

Fertil Steril. 2012 Mar 7. [Epub ahead of print]

Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence.

Minasi MG, Fabozzi G, Casciani V, Ferrero S, Litwicka K, Greco E.

Source

Center For Reproductive Medicine, European Hospital, Rome, Italy.

Abstract

OBJECTIVE:

To evaluate the efficiency of slush nitrogen vitrification of human oocytes with or without cumulus cells in terms of survival rate and maintenance of meiotic spindle.

DESIGN:

Randomized, comparative study.

SETTING:

Medical center.

PATIENT(S):

A total of 274 oocytes obtained from 46 couples undergoing infertility treatment.

INTERVENTION(S):

Metaphase II oocytes were divided into groups A and B, vitrified with and without cumulus cells, respectively.

MAIN OUTCOMES MEASURE(S):

Survival rates and maintenance of meiotic spindle observed immediately after warming and 3 hours after incubation.

RESULT(S):

No statistically significant difference was detected between the two groups in terms of survival rate, but a significantly higher percentage of detectable spindle was observed in group B (completely denuded oocytes), either immediately after warming or 3 hours after incubation.

CONCLUSION(S):

Complete denudation of oocytes before slush nitrogen vitrification does not influence survival rates but positively affects oocyte meiotic spindle competence. These data support the hypothesis that cumulus cells duringvitrification represent an obstacle to cryoprotectant penetration more than having a protective role for the oocyte.