2012年1月18日

白蛋白XY精蟲分離法

將清洗後精蟲至於18%-8%白蛋白之離心管上層
靜置1小時候Y精蟲會游到18%白蛋白層
蒐集18%白蛋白層&再次離心分離
http://www.in-gender.com/Gender-Selection/Ericsson/Ericsson.aspx


Sperm Preparation by the Ericsson Albumin Method

The Ericsson method is based on the assumption that Y-sperm swim faster than X-sperm. Sperm are placed in a test tube atop a "column" of increasingly thicker layers of albumin, and allowed to swim down into the solution. After a certain time period has elapsed, only the fastest sperm should have been able to penetrate to the bottom layer. Here is the procedure as described by Dr. Scott Ericsson in one study:Ericsson method for gender selection
  • Human serum albumin (HSA), a sticky protein solution, is layered into a column of increasing thickness, here of 7.5% and 17.5%. (Note: This figure is for illustration only, I don't know what color albumin really is.)
  • After the semen is centrifuged, washed, and diluted, it is layered on top of the albumin column.
  • The column is allowed to stand for 1 hour, allowing sperm to penetrate the albumin. (Although the semen is processed in a centrifuge during the procedure, it is this step -- allowing the sperm to swim through the albumin column -- that it intended to separate X and Y sperm, rather than the spinning.)
  • The top layer is discarded, and the column is allowed to stand for another 30 minutes.
  • The next layer (originally the middle layer) is discarded.
  • The remainding bottom layer is centrifuged, and of this only the pellet (sediment at the bottom) is retained and prepared for artificial insemination.

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