上游法為人工授精IUI之優先使用方法

人工授精(IUI)懷孕率約10-14%
其中Percoll 分離法懷孕率最高: 14% (目前臨床禁用)
建議常規使用上游法為IUI之優先使用方法

Arch Androl. 2004 May-Jun;50(3):139-43.

Comparison of four methods for sperm preparation for IUI.

Ren SS, Sun GH, Ku CH, Chen DC, Wu GJ.

Source

National Defense Medical Center, National Defense University, Taipei, Taiwan, ROC.

Abstract

We compared the pregnancy rates and sperm characteristics of semen prepared by the albumin, Percoll, Puresperm, and swim-up methods. Semen analysis was performed by a computer-assisted system (CASA). The overall pregnancy rate was 14% per patient and 11% per cycle. The albumin and Percoll methods had the highest pregnancy rates, up to 12% per cycle. We then compared each method with the albumin method. The swim-up technique yielded the highest percentage of motile sperm. The Percoll method yielded statistically significant changes in average path velocity and straight-line velocity, while the Puresperm method revealed the same statistical changes in total concentration and hyperactive motile spermpercentage. After adjusting for age, methods, and CASA estimates, only straight-line velocity was significantly correlated with pregnancy. Although the Percoll method was associated with the best pregnancy rate, this product has been withdrawn from the market because of toxic contamination. Puresperm had replaced it in our laboratory but showed an unsatisfactory pregnancy rate. The swim-up method is the best choice for IUI.