不同胚胎輔助孵化方法對胚胎植入懷孕率無明顯差異

本篇比較不同胚胎輔助孵化之方法包括中空透明帶打薄或打洞對於胚胎植入後懷孕率之影響
結論並無明顯差異

http://humrep.oxfordjournals.org/content/17/5/1239.full

A comparison of four different techniques of assisted hatching

Table II.

Patient characteristics and cycle outcome with different methods of assisted hatching

	Group 1 Partial zona dissection	Group 2 Acid tyrode	Group 3 Diode laser	Group 4 Pronase zona thinning	Group 5 Controls
aThe control group is significantly different from the other groups (analysis of variance with Tukey post-hoc test, P < 0.05).
No. of embryo transfer cycles	239	191	219	145	188
Mean ± SD age (years)a	34.4 ± 3.3	34.6 ±3.2	35.0 ±3.3	34.5 ±3.1	29.8 ±3.0
Mean ± SD no. of previous assisted reproduction cyclesa	1.9 ± 0.6	1.7 ±0.5	2.1 ±0.8	2.1 ±0.7	0
Mean ± SD basal FSH (mIU/ml)a	6.9 ± 1.1	7.1 ±1.2	7.4 ±1.3	7.1 ±1.3	3.9 ± 1.0
No. of oocytes retrieved	2295	1872	2081	1407	2313
(mean ± SD)a	(9.6 ±2.2)	(9.8 ±2.2)	(9.5 ±2.1)	(9.7 ±2.2)	(12.3 ±3.0)
Oocytes injected	1791	1445	1624	1126	1783
2-Pronuclear fertilization rate (%)	70	69	70	70	69.9
Degeneration rate (%)	3.6	4.0	3.3	3.9	3.1
Cleavage rate (%)	98	98.4	98	98.2	98
No. (%) G1 + G2 embryos	702 (57)	574 (58.3)	653 (58.5)	455 (59.0)	795 (65)
Zona thickness (μm)a	14.4 ± 2.0	14.9 ±2.0	14.8 ±2.0	14.3 ±2.1	12.1 ±1.9
No. of embryos transferred	932	763	876	537	652
(mean ± SD)	(3.8 ±1.1)	(3.9 ±1.0)	(4.0 ±0.9)	(3.7 ±1.1)	(3.4 ±0.7)
Implantation/embryo (%)	174/932	133/763	166/876	103/537	142/652
	(18.6)	(17.4)	(18.9)	(19.1)	(21.6)
Clinical pregnancy/embryo transfer (%)	118/239	88/191	106/219	68/145	91/188
	(49.3)	(46.0)	(48.4)	(46.8)	(48.4)
Multiple pregnancy rate (%)	50.8	45.4	50.0	48.5	45.1
Singletons/twins/triplets	46/60/12	48/35/5	53/46/7	35/31/2	50/31/10
Abortion rate (%)	18.6	20.4	19.8	17.6	16.