上游法或分層離心法對精蟲XY性別並無顯著影響

本篇顯示上游法或分層離心法並無顯著影響精蟲XY之比例

Fertil Steril. 1993 Feb;59(2):387-92.

The sex ratio of normal and manipulated human sperm quantitated by the polymerase chain reaction.

Lobel SM, Pomponio RJ, Mutter GL.

Source

Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, Massachusetts 02115.

Abstract

OBJECTIVE:

To establish the primary sex ratio, the relative abundance of X and Y chromosome-bearing sperm, in unselected sperm and in sperm selected by swim-up or Sephadex filtration (SpermPrep column; Fertility Technologies, Inc., Natick, MA). This was done to evaluate the possibility that these semen manipulations change the primary sex ratio.

DESIGN:

Ninety-eight unmanipulated semen samples were analyzed for sex chromosome content using quantitative polymerase chain reaction (PCR). A smaller number of samples was analyzed before and after either swim-up or Sephadex filtration.

RESULTS:

The mean percentage of all sex chromosomes identified as a Y chromosome in unmanipulated semen samples ranged from 41.9% to 56.7%, with a mean average of 50.3%. There was no significant change in sex chromosome composition after either swim-up (n = 17) or column filtration (n = 20).

CONCLUSIONS:

The chromosome compositions of semen samples from a large number of men have equal numbers of X and Y. Swim-up and SpermPrep filtration do not appear to alter the primary sex ratio.