Ficoll-400 密度梯度可用於精蟲分離&產生了更高百分比的活的、成熟的、形態正常的精子。

分層方式: 5% (v/v) density top layer and 90% (v/v) Ficoll-400 density lower layer. 

密度比較: Ficoll density 1.2 g/ml

-----normal spermatozoa (1.17 g/ml)

-----immotile immature sperm cells having lower density of 1.02 g/ml,

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Ficoll-400 density gradient method as an effective sperm preparation technique for assisted reproductive techniques J Hum Reprod Sci. 2016 Jul-Sep; 9(3): 194–199.

Ficoll-400 density gradient yielded higher percentage of live, mature, morphologically normal spermatozoa in an isolated fraction as compared to other three techniques.