使用密度梯度 density gradient centrifugation來分離狗精子性別的技術並不有效。

. 2018 Dec:199:84-92.  

Sperm sexing with density gradient centrifugation in dogs

•  Sperms were separated using gradients of Percoll® or Ficoll. 
• The percentage of sperm containing X and Y chromosomes was evaluated by qPCR. 
• The use of the Ficoll gradient resulted in the greatest sperm quality after centrifugation. 
• No sperm enhancement containing X or Y chromosome occurred with use of any of the methods 

1. Percoll® 54.8 ± 1.9 compared with 45.2 ± 1.9; 
2. Percoll® associated with Nycodenz® 53.2 ± 2.0 compared with 46.8 ± 2.0; 
3. Ficoll 55.0 ± 1.5 compared with 45.0 ± 1.5 
4. for the percentages of cells containing the X and Y chromosomes, respectively

• The technique of sexing dog sperm using density gradients was not effective for commercial application.